Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3624476..3625170 Replicon chromosome
Accession NZ_CP103570
Organism Escherichia coli strain 3045

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5S79_RS17470 Protein ID WP_001263489.1
Coordinates 3624476..3624874 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5S79_RS17475 Protein ID WP_000554758.1
Coordinates 3624877..3625170 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3620064) 3620064..3620144 - 81 NuclAT_11 - -
- (3620064) 3620064..3620144 - 81 NuclAT_11 - -
- (3620064) 3620064..3620144 - 81 NuclAT_11 - -
- (3620064) 3620064..3620144 - 81 NuclAT_11 - -
M5S79_RS17445 (3620740) 3620740..3621198 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5S79_RS17450 (3621459) 3621459..3622916 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5S79_RS17455 (3622973) 3622973..3623494 - 522 Protein_3413 peptide chain release factor H -
M5S79_RS17460 (3623490) 3623490..3623696 - 207 Protein_3414 RtcB family protein -
M5S79_RS17465 (3624014) 3624014..3624466 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5S79_RS17470 (3624476) 3624476..3624874 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5S79_RS17475 (3624877) 3624877..3625170 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5S79_RS17480 (3625222) 3625222..3626277 - 1056 WP_001226164.1 DNA polymerase IV -
M5S79_RS17485 (3626348) 3626348..3627133 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5S79_RS17490 (3627105) 3627105..3628817 + 1713 Protein_3420 flagellar biosynthesis protein FlhA -
M5S79_RS17495 (3629041) 3629041..3629538 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3624476..3639756 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T255619 WP_001263489.1 NZ_CP103570:c3624874-3624476 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255619 WP_000554758.1 NZ_CP103570:c3625170-3624877 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References