Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1975895..1976397 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag M5R24_RS09435 Protein ID WP_000767819.1
Coordinates 1976143..1976397 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag M5R24_RS09430 Protein ID WP_001259253.1
Coordinates 1975895..1976146 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS09405 (1971073) 1971073..1972140 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
M5R24_RS09410 (1972140) 1972140..1973210 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
M5R24_RS09415 (1973207) 1973207..1974511 - 1305 WP_001296211.1 histidinol dehydrogenase -
M5R24_RS09420 (1974517) 1974517..1975416 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
M5R24_RS09425 (1975562) 1975562..1975612 - 51 WP_001364200.1 his operon leader peptide -
M5R24_RS09430 (1975895) 1975895..1976146 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
M5R24_RS09435 (1976143) 1976143..1976397 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
M5R24_RS09440 (1976480) 1976480..1977304 + 825 WP_000754737.1 SDR family oxidoreductase -
M5R24_RS09445 (1977350) 1977350..1978279 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
M5R24_RS09450 (1978494) 1978494..1978556 + 63 WP_010723108.1 membrane protein YoeI -
M5R24_RS09455 (1978546) 1978546..1979904 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
M5R24_RS09460 (1980121) 1980121..1980579 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1980121..1980579 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T255562 WP_000767819.1 NZ_CP103562:1976143-1976397 [Escherichia coli O25b:H4-ST131]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT255562 WP_001259253.1 NZ_CP103562:1975895-1976146 [Escherichia coli O25b:H4-ST131]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References