Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 642877..643676 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag M5R24_RS03175 Protein ID WP_000347251.1
Coordinates 642877..643341 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag M5R24_RS03180 Protein ID WP_001296435.1
Coordinates 643341..643676 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS03145 (637878) 637878..638312 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5R24_RS03150 (638330) 638330..639208 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5R24_RS03155 (639198) 639198..639977 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5R24_RS03160 (639988) 639988..640461 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5R24_RS03165 (640484) 640484..641764 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5R24_RS03170 (642013) 642013..642822 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5R24_RS03175 (642877) 642877..643341 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5R24_RS03180 (643341) 643341..643676 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5R24_RS03185 (643825) 643825..645396 - 1572 WP_001273763.1 galactarate dehydratase -
M5R24_RS03190 (645771) 645771..647105 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5R24_RS03195 (647121) 647121..647891 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T255558 WP_000347251.1 NZ_CP103562:c643341-642877 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT255558 WP_001296435.1 NZ_CP103562:c643676-643341 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References