Detailed information of TA system TA025554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3607580..3608250
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G0THF5
• Locus tag: MCAN_RS16825
• Protein ID: WP_014001612.1
• Coordinates: 3607580..3607924 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: MCAN_RS16830
• Protein ID: WP_003899955.1
• Coordinates: 3607921..3608250 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS16795	3602653..3603513	+	861	WP_014001610.1	alpha/beta hydrolase	-
MCAN_RS16800	3603488..3604003	+	516	WP_015288470.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MCAN_RS16805	3604243..3604536	+	294	WP_003416635.1	hypothetical protein	-
MCAN_RS16810	3604824..3606113	+	1290	WP_003416640.1	ATP-binding protein	-
MCAN_RS16815	3606460..3606894	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS16820	3606897..3607349	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MCAN_RS16825	3607580..3607924	+	345	WP_014001612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCAN_RS16830	3607921..3608250	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
MCAN_RS16835	3608788..3609135	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
MCAN_RS16840	3609132..3609755	+	624	WP_014001613.1	RES domain-containing protein	-
MCAN_RS16850	3609878..3611139	+	1262	WP_145974184.1	IS3-like element IS987 family transposase	-
MCAN_RS16855	3611186..3611959	+	774	WP_014001615.1	hypothetical protein	-
MCAN_RS16860	3611986..3613020	-	1035	WP_014001616.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12752.60 Da
• Isoelectric Point: 5.6920

>T25554 WP_014001612.1 NC_015848:3607580-3607924 [Mycobacterium canettii CIPT 140010059]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRFENWLASEHGGG

Nucleotide

• Length: 345 bp

>T25554 NC_015848:3607580-3607924 [Mycobacterium canettii CIPT 140010059]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTTCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT25554 WP_003899955.1 NC_015848:3607921-3608250 [Mycobacterium canettii CIPT 140010059]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT25554 NC_015848:3607921-3608250 [Mycobacterium canettii CIPT 140010059]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACCGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: G0THF5

Antitoxin

• AlphaFold DB: G0THF6