Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5116035..5116637 Replicon chromosome
Accession NZ_CP103559
Organism Escherichia coli strain 3059

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5T18_RS24870 Protein ID WP_000897305.1
Coordinates 5116326..5116637 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5T18_RS24865 Protein ID WP_000356395.1
Coordinates 5116035..5116325 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T18_RS24830 (5111659) 5111659..5112561 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5T18_RS24835 (5112558) 5112558..5113193 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5T18_RS24840 (5113190) 5113190..5114119 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
M5T18_RS24845 (5114301) 5114301..5114543 - 243 WP_001309881.1 CopG family transcriptional regulator -
M5T18_RS24850 (5114762) 5114762..5114980 - 219 WP_001295676.1 CopG family transcriptional regulator -
M5T18_RS24855 (5115399) 5115399..5115677 - 279 WP_001315112.1 hypothetical protein -
M5T18_RS24860 (5115729) 5115729..5115950 - 222 WP_001550354.1 hypothetical protein -
M5T18_RS24865 (5116035) 5116035..5116325 - 291 WP_000356395.1 NadS family protein Antitoxin
M5T18_RS24870 (5116326) 5116326..5116637 - 312 WP_000897305.1 hypothetical protein Toxin
M5T18_RS24875 (5116866) 5116866..5117774 + 909 WP_001550353.1 alpha/beta hydrolase -
M5T18_RS24880 (5117942) 5117942..5118856 - 915 WP_109553727.1 transposase -
M5T18_RS24885 (5118869) 5118869..5119756 - 888 Protein_4863 hypothetical protein -
M5T18_RS24890 (5120172) 5120172..5121113 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5T18_RS24895 (5121158) 5121158..5121595 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T255539 WP_000897305.1 NZ_CP103559:c5116637-5116326 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT255539 WP_000356395.1 NZ_CP103559:c5116325-5116035 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References