Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1169207..1169934 Replicon chromosome
Accession NZ_CP103559
Organism Escherichia coli strain 3059

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag M5T18_RS05650 Protein ID WP_000547555.1
Coordinates 1169207..1169518 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5T18_RS05655 Protein ID WP_000126294.1
Coordinates 1169515..1169934 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T18_RS05625 (1165142) 1165142..1166851 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
M5T18_RS05630 (1166861) 1166861..1167403 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
M5T18_RS05635 (1167403) 1167403..1168170 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
M5T18_RS05640 (1168167) 1168167..1168577 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
M5T18_RS05645 (1168570) 1168570..1169040 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
M5T18_RS05650 (1169207) 1169207..1169518 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
M5T18_RS05655 (1169515) 1169515..1169934 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
M5T18_RS05660 (1170013) 1170013..1171437 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
M5T18_RS05665 (1171446) 1171446..1172903 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
M5T18_RS05670 (1173163) 1173163..1174173 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
M5T18_RS05675 (1174322) 1174322..1174849 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T255514 WP_000547555.1 NZ_CP103559:1169207-1169518 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT255514 WP_000126294.1 NZ_CP103559:1169515-1169934 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References