Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3824174..3824853 Replicon chromosome
Accession NZ_CP103557
Organism Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M5T49_RS19295 Protein ID WP_000057523.1
Coordinates 3824551..3824853 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5T49_RS19290 Protein ID WP_000806442.1
Coordinates 3824174..3824515 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T49_RS19280 (3820418) 3820418..3821350 - 933 WP_000883041.1 glutaminase A -
M5T49_RS19285 (3821612) 3821612..3824116 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
M5T49_RS19290 (3824174) 3824174..3824515 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5T49_RS19295 (3824551) 3824551..3824853 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T49_RS19300 (3824986) 3824986..3825780 + 795 WP_000365147.1 TraB/GumN family protein -
M5T49_RS19305 (3825984) 3825984..3826463 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5T49_RS19310 (3826631) 3826631..3827287 + 657 WP_015674862.1 hypothetical protein -
M5T49_RS19315 (3827284) 3827284..3827787 + 504 WP_000667000.1 hypothetical protein -
M5T49_RS19320 (3827825) 3827825..3829477 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T255495 WP_000057523.1 NZ_CP103557:c3824853-3824551 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT255495 WP_000806442.1 NZ_CP103557:c3824515-3824174 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References