Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2018306..2018808 Replicon chromosome
Accession NZ_CP103557
Organism Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag M5T49_RS09770 Protein ID WP_000767819.1
Coordinates 2018554..2018808 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag M5T49_RS09765 Protein ID WP_001259253.1
Coordinates 2018306..2018557 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T49_RS09740 (2013484) 2013484..2014551 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
M5T49_RS09745 (2014551) 2014551..2015621 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
M5T49_RS09750 (2015618) 2015618..2016922 - 1305 WP_001296211.1 histidinol dehydrogenase -
M5T49_RS09755 (2016928) 2016928..2017827 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
M5T49_RS09760 (2017973) 2017973..2018023 - 51 WP_001364200.1 his operon leader peptide -
M5T49_RS09765 (2018306) 2018306..2018557 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
M5T49_RS09770 (2018554) 2018554..2018808 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
M5T49_RS09775 (2018891) 2018891..2019715 + 825 WP_000754737.1 SDR family oxidoreductase -
M5T49_RS09780 (2019761) 2019761..2020690 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
M5T49_RS09785 (2020905) 2020905..2020967 + 63 WP_010723108.1 membrane protein YoeI -
M5T49_RS09790 (2020957) 2020957..2022315 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
M5T49_RS09795 (2022532) 2022532..2022990 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2022532..2022990 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T255488 WP_000767819.1 NZ_CP103557:2018554-2018808 [Escherichia coli O25b:H4-ST131]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT255488 WP_001259253.1 NZ_CP103557:2018306-2018557 [Escherichia coli O25b:H4-ST131]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References