Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40525..41175 Replicon chromosome
Accession NZ_CP103554
Organism Klebsiella michiganensis strain 5088

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag M5R35_RS00195 Protein ID WP_014227314.1
Coordinates 40525..40866 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag M5R35_RS00200 Protein ID WP_014227313.1
Coordinates 40876..41175 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R35_RS00180 (M5R35_00180) 36517..37836 + 1320 WP_014227316.1 MFS transporter -
M5R35_RS00185 (M5R35_00185) 37982..39373 + 1392 WP_014227315.1 hexose-6-phosphate:phosphate antiporter -
M5R35_RS00190 (M5R35_00190) 39822..40274 + 453 WP_009653860.1 DUF1198 domain-containing protein -
M5R35_RS00195 (M5R35_00195) 40525..40866 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5R35_RS00200 (M5R35_00200) 40876..41175 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
M5R35_RS00205 (M5R35_00205) 41294..42475 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
M5R35_RS00210 (M5R35_00210) 42508..43317 - 810 WP_038422921.1 phosphonoacetaldehyde hydrolase -
M5R35_RS00215 (M5R35_00215) 43327..44430 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
M5R35_RS00220 (M5R35_00220) 44571..45290 + 720 WP_014227309.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T255467 WP_014227314.1 NZ_CP103554:40525-40866 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT255467 WP_014227313.1 NZ_CP103554:40876-41175 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References