Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 1527868..1528557 Replicon chromosome
Accession NZ_CP103548
Organism Klebsiella pneumoniae strain 3987

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag M5T27_RS07420 Protein ID WP_021469727.1
Coordinates 1528240..1528557 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag M5T27_RS07415 Protein ID WP_020804705.1
Coordinates 1527868..1528164 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T27_RS07400 (1524260) 1524260..1525642 + 1383 WP_004151222.1 MFS transporter -
M5T27_RS07405 (1525686) 1525686..1527302 + 1617 WP_004175961.1 carbohydrate porin -
M5T27_RS07410 (1527343) 1527343..1527789 - 447 WP_032435212.1 hypothetical protein -
M5T27_RS07415 (1527868) 1527868..1528164 - 297 WP_020804705.1 NadS family protein Antitoxin
M5T27_RS07420 (1528240) 1528240..1528557 - 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T27_RS07425 (1528764) 1528764..1528991 - 228 WP_002906690.1 tautomerase PptA -
M5T27_RS07430 (1529062) 1529062..1529679 - 618 WP_032425015.1 glutathione S-transferase family protein -
M5T27_RS07435 (1529757) 1529757..1530608 - 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
M5T27_RS07440 (1530647) 1530647..1531426 - 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
M5T27_RS07445 (1531410) 1531410..1532336 - 927 WP_032435210.1 amino acid ABC transporter permease -
M5T27_RS07450 (1532346) 1532346..1532855 - 510 WP_020802144.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T255436 WP_021469727.1 NZ_CP103548:c1528557-1528240 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT255436 WP_020804705.1 NZ_CP103548:c1528164-1527868 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References