Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3701112..3701653 Replicon chromosome
Accession NZ_CP103544
Organism Escherichia coli strain 3036

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag M5S54_RS17980 Protein ID WP_000615976.1
Coordinates 3701375..3701653 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M5S54_RS17975 Protein ID WP_000729704.1
Coordinates 3701112..3701372 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S54_RS17950 (3696895) 3696895..3697680 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
M5S54_RS17955 (3697652) 3697652..3699364 + 1713 Protein_3514 flagellar biosynthesis protein FlhA -
M5S54_RS17960 (3699469) 3699469..3699747 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
M5S54_RS17965 (3699740) 3699740..3700096 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
M5S54_RS17970 (3700153) 3700153..3700926 - 774 WP_000543895.1 C40 family peptidase -
M5S54_RS17975 (3701112) 3701112..3701372 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5S54_RS17980 (3701375) 3701375..3701653 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5S54_RS17985 (3701809) 3701809..3702549 + 741 WP_063100007.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5S54_RS17990 (3702520) 3702520..3703287 - 768 WP_000333380.1 class II glutamine amidotransferase -
M5S54_RS17995 (3703493) 3703493..3704071 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3699740..3712439 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-91)

Antitoxin

(4-84)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T255414 WP_000615976.1 NZ_CP103544:3701375-3701653 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp


Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT255414 WP_000729704.1 NZ_CP103544:3701112-3701372 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U9XPG0


Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References