Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3701066..3701760 Replicon chromosome
Accession NZ_CP103540
Organism Escherichia coli strain 5264

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5S66_RS17960 Protein ID WP_001263489.1
Coordinates 3701066..3701464 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5S66_RS17965 Protein ID WP_000554758.1
Coordinates 3701467..3701760 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3696654) 3696654..3696734 - 81 NuclAT_13 - -
- (3696654) 3696654..3696734 - 81 NuclAT_13 - -
- (3696654) 3696654..3696734 - 81 NuclAT_13 - -
- (3696654) 3696654..3696734 - 81 NuclAT_13 - -
M5S66_RS17935 (3697330) 3697330..3697788 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5S66_RS17940 (3698049) 3698049..3699506 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5S66_RS17945 (3699563) 3699563..3700084 - 522 Protein_3507 peptide chain release factor H -
M5S66_RS17950 (3700080) 3700080..3700286 - 207 Protein_3508 RtcB family protein -
M5S66_RS17955 (3700604) 3700604..3701056 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5S66_RS17960 (3701066) 3701066..3701464 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5S66_RS17965 (3701467) 3701467..3701760 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5S66_RS17970 (3701812) 3701812..3702867 - 1056 WP_001226164.1 DNA polymerase IV -
M5S66_RS17975 (3702938) 3702938..3703723 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5S66_RS17980 (3703695) 3703695..3705407 + 1713 Protein_3514 flagellar biosynthesis protein FlhA -
M5S66_RS17985 (3705631) 3705631..3706128 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T255391 WP_001263489.1 NZ_CP103540:c3701464-3701066 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255391 WP_000554758.1 NZ_CP103540:c3701760-3701467 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References