255379

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 647134..647933 Replicon chromosome
Accession NZ_CP103540
Organism Escherichia coli strain 5264

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag M5S66_RS03160 Protein ID WP_000347272.1
Coordinates 647134..647598 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5S66_RS03165 Protein ID WP_001307405.1
Coordinates 647598..647933 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S66_RS03130 (642135) 642135..642569 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S66_RS03135 (642587) 642587..643465 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S66_RS03140 (643455) 643455..644234 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S66_RS03145 (644245) 644245..644718 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S66_RS03150 (644741) 644741..646021 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S66_RS03155 (646270) 646270..647079 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
M5S66_RS03160 (647134) 647134..647598 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S66_RS03165 (647598) 647598..647933 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S66_RS03170 (648082) 648082..649653 - 1572 WP_001273738.1 galactarate dehydratase -
M5S66_RS03175 (650028) 650028..651362 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5S66_RS03180 (651378) 651378..652148 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 647134..658586 11452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T255379 WP_000347272.1 NZ_CP103540:c647598-647134 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255379 WP_001307405.1 NZ_CP103540:c647933-647598 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References