Detailed information of TA system TA025537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2316856..2317553
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: MCAN_RS10685
• Protein ID: WP_178125313.1
• Coordinates: 2317170..2317553 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: G0TM15
• Locus tag: MCAN_RS10680
• Protein ID: WP_014001072.1
• Coordinates: 2316856..2317161 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS21475	2312415..2313342	-	928	Protein_2095	IS5 family transposase	-
MCAN_RS10655	2313475..2314050	+	576	WP_014001068.1	hypothetical protein	-
MCAN_RS10660	2314047..2315087	+	1041	WP_014001069.1	XRE family transcriptional regulator	-
MCAN_RS10665	2315329..2316048	+	720	WP_014001070.1	DUF433 domain-containing protein	-
MCAN_RS10670	2316038..2316451	+	414	Protein_2099	hypothetical protein	-
MCAN_RS10675	2316470..2316770	-	301	Protein_2100	hypothetical protein	-
MCAN_RS10680	2316856..2317161	-	306	WP_014001072.1	helix-turn-helix transcriptional regulator	Antitoxin
MCAN_RS10685	2317170..2317553	-	384	WP_178125313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCAN_RS10690	2318058..2318432	+	375	Protein_2103	AAA family ATPase	-
MCAN_RS10695	2318602..2320008	-	1407	WP_014001075.1	IS1380 family transposase	-
MCAN_RS10700	2320532..2321530	-	999	WP_014001076.1	cation diffusion facilitator family transporter	-
MCAN_RS10705	2321645..2322529	-	885	WP_014001077.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2318602..2320008	1406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-88)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14491.55 Da
• Isoelectric Point: 10.1259

>T25537 WP_178125313.1 NC_015848:c2317553-2317170 [Mycobacterium canettii CIPT 140010059]
VGWTADVERISAWLNGLDRETYELVFAAIEVLEEEGPALGRPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQA
IMLAAGNKAGRWSQWYHEKIKAADEMFAEHLAQFEDTKRKKRKPKKG

Nucleotide

• Length: 384 bp

>T25537 NC_015848:c2317553-2317170 [Mycobacterium canettii CIPT 140010059]
GTGGGGTGGACGGCGGATGTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGC
AGCTATCGAGGTTCTCGAGGAAGAGGGCCCCGCACTTGGCCGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGA
ACATGAAGGAGCTGCGTCCAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCG
ATCATGCTGGCCGCCGGCAACAAGGCCGGGCGGTGGTCCCAGTGGTACCACGAGAAGATCAAAGCGGCCGATGAGATGTT
TGCCGAACATCTCGCACAGTTCGAGGACACGAAGCGAAAGAAGCGCAAGCCCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11363.97 Da
• Isoelectric Point: 10.1820

>AT25537 WP_014001072.1 NC_015848:c2317161-2316856 [Mycobacterium canettii CIPT 140010059]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAELAHIRQSRVSSIENGDIGTAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT25537 NC_015848:c2317161-2316856 [Mycobacterium canettii CIPT 140010059]
ATGGCGATGACACTGCGGGATATGGACGCCGTACGTCCCGTCAACCGGGAGGCGGTGGACCGACACAAGGCGCGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCCGAGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCACCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Antitoxin

• AlphaFold DB: G0TM15