Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3939342..3940036 Replicon chromosome
Accession NZ_CP103529
Organism Escherichia coli strain 5270

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5T24_RS19080 Protein ID WP_001263489.1
Coordinates 3939342..3939740 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5T24_RS19085 Protein ID WP_000554758.1
Coordinates 3939743..3940036 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3934930) 3934930..3935010 - 81 NuclAT_13 - -
- (3934930) 3934930..3935010 - 81 NuclAT_13 - -
- (3934930) 3934930..3935010 - 81 NuclAT_13 - -
- (3934930) 3934930..3935010 - 81 NuclAT_13 - -
M5T24_RS19055 (3935606) 3935606..3936064 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5T24_RS19060 (3936325) 3936325..3937782 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5T24_RS19065 (3937839) 3937839..3938360 - 522 Protein_3734 peptide chain release factor H -
M5T24_RS19070 (3938356) 3938356..3938562 - 207 Protein_3735 RtcB family protein -
M5T24_RS19075 (3938880) 3938880..3939332 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5T24_RS19080 (3939342) 3939342..3939740 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T24_RS19085 (3939743) 3939743..3940036 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T24_RS19090 (3940088) 3940088..3941143 - 1056 WP_001226164.1 DNA polymerase IV -
M5T24_RS19095 (3941214) 3941214..3941999 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5T24_RS19100 (3941971) 3941971..3943683 + 1713 Protein_3741 flagellar biosynthesis protein FlhA -
M5T24_RS19105 (3943907) 3943907..3944404 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T255341 WP_001263489.1 NZ_CP103529:c3939740-3939342 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255341 WP_000554758.1 NZ_CP103529:c3940036-3939743 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References