Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4910324..4910926 Replicon chromosome
Accession NZ_CP103521
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5S63_RS23770 Protein ID WP_000897305.1
Coordinates 4910615..4910926 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S63_RS23765 Protein ID WP_000356395.1
Coordinates 4910324..4910614 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS23730 (4905947) 4905947..4906849 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5S63_RS23735 (4906846) 4906846..4907481 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5S63_RS23740 (4907478) 4907478..4908407 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
M5S63_RS23745 (4908589) 4908589..4908831 - 243 WP_021523315.1 hypothetical protein -
M5S63_RS23750 (4909050) 4909050..4909268 - 219 WP_001295676.1 CopG family transcriptional regulator -
M5S63_RS23755 (4909687) 4909687..4909965 - 279 WP_001306650.1 hypothetical protein -
M5S63_RS23760 (4910027) 4910027..4910239 - 213 WP_000197769.1 hypothetical protein -
M5S63_RS23765 (4910324) 4910324..4910614 - 291 WP_000356395.1 NadS family protein Antitoxin
M5S63_RS23770 (4910615) 4910615..4910926 - 312 WP_000897305.1 hypothetical protein Toxin
M5S63_RS23775 (4911155) 4911155..4912063 + 909 WP_001306651.1 alpha/beta hydrolase -
M5S63_RS23780 (4912232) 4912232..4913146 - 915 WP_077634137.1 transposase -
M5S63_RS23785 (4913159) 4913159..4914046 - 888 Protein_4649 hypothetical protein -
M5S63_RS23790 (4914462) 4914462..4915403 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5S63_RS23795 (4915448) 4915448..4915885 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T255313 WP_000897305.1 NZ_CP103521:c4910926-4910615 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT255313 WP_000356395.1 NZ_CP103521:c4910614-4910324 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References