Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 624583..625382 Replicon chromosome
Accession NZ_CP103521
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag M5S63_RS02990 Protein ID WP_000347272.1
Coordinates 624583..625047 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5S63_RS02995 Protein ID WP_001307405.1
Coordinates 625047..625382 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS02960 (619584) 619584..620018 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S63_RS02965 (620036) 620036..620914 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S63_RS02970 (620904) 620904..621683 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S63_RS02975 (621694) 621694..622167 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S63_RS02980 (622190) 622190..623470 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S63_RS02985 (623719) 623719..624528 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
M5S63_RS02990 (624583) 624583..625047 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S63_RS02995 (625047) 625047..625382 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S63_RS03000 (625531) 625531..627102 - 1572 WP_001273738.1 galactarate dehydratase -
M5S63_RS03005 (627477) 627477..628811 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5S63_RS03010 (628827) 628827..629597 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 624583..636034 11451


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T255289 WP_000347272.1 NZ_CP103521:c625047-624583 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255289 WP_001307405.1 NZ_CP103521:c625382-625047 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References