Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3661582..3662123 Replicon chromosome
Accession NZ_CP103515
Organism Escherichia coli strain 5444

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M5S96_RS17985 Protein ID WP_000615983.1
Coordinates 3661845..3662123 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M5S96_RS17980 Protein ID WP_000729703.1
Coordinates 3661582..3661842 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S96_RS17960 (3657257) 3657257..3658042 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5S96_RS17965 (3658014) 3658014..3659726 + 1713 Protein_3514 flagellar biosynthesis protein FlhA -
M5S96_RS17970 (3659950) 3659950..3660447 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M5S96_RS17975 (3660623) 3660623..3661372 - 750 WP_000056849.1 C40 family peptidase -
M5S96_RS17980 (3661582) 3661582..3661842 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5S96_RS17985 (3661845) 3661845..3662123 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5S96_RS17990 (3662279) 3662279..3663019 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5S96_RS17995 (3662990) 3662990..3663757 - 768 WP_000333380.1 class II glutamine amidotransferase -
M5S96_RS18000 (3663963) 3663963..3664541 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3655385..3670665 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T255276 WP_000615983.1 NZ_CP103515:3661845-3662123 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT255276 WP_000729703.1 NZ_CP103515:3661582-3661842 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References