Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3655385..3656079 Replicon chromosome
Accession NZ_CP103515
Organism Escherichia coli strain 5444

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5S96_RS17945 Protein ID WP_001263489.1
Coordinates 3655385..3655783 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5S96_RS17950 Protein ID WP_000554758.1
Coordinates 3655786..3656079 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3650973) 3650973..3651053 - 81 NuclAT_11 - -
- (3650973) 3650973..3651053 - 81 NuclAT_11 - -
- (3650973) 3650973..3651053 - 81 NuclAT_11 - -
- (3650973) 3650973..3651053 - 81 NuclAT_11 - -
M5S96_RS17920 (3651649) 3651649..3652107 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5S96_RS17925 (3652368) 3652368..3653825 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5S96_RS17930 (3653882) 3653882..3654403 - 522 Protein_3507 peptide chain release factor H -
M5S96_RS17935 (3654399) 3654399..3654605 - 207 Protein_3508 RtcB family protein -
M5S96_RS17940 (3654923) 3654923..3655375 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5S96_RS17945 (3655385) 3655385..3655783 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5S96_RS17950 (3655786) 3655786..3656079 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5S96_RS17955 (3656131) 3656131..3657186 - 1056 WP_001226164.1 DNA polymerase IV -
M5S96_RS17960 (3657257) 3657257..3658042 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5S96_RS17965 (3658014) 3658014..3659726 + 1713 Protein_3514 flagellar biosynthesis protein FlhA -
M5S96_RS17970 (3659950) 3659950..3660447 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3655385..3670665 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T255275 WP_001263489.1 NZ_CP103515:c3655783-3655385 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255275 WP_000554758.1 NZ_CP103515:c3656079-3655786 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References