Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603489..604288 Replicon chromosome
Accession NZ_CP103508
Organism Escherichia coli O25b:H4-ST131 strain 5506

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag M5T03_RS02975 Protein ID WP_000347251.1
Coordinates 603489..603953 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag M5T03_RS02980 Protein ID WP_001296435.1
Coordinates 603953..604288 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T03_RS02945 (598490) 598490..598924 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T03_RS02950 (598942) 598942..599820 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T03_RS02955 (599810) 599810..600589 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T03_RS02960 (600600) 600600..601073 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T03_RS02965 (601096) 601096..602376 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T03_RS02970 (602625) 602625..603434 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5T03_RS02975 (603489) 603489..603953 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T03_RS02980 (603953) 603953..604288 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T03_RS02985 (604437) 604437..606008 - 1572 WP_001273763.1 galactarate dehydratase -
M5T03_RS02990 (606383) 606383..607717 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5T03_RS02995 (607733) 607733..608503 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T255216 WP_000347251.1 NZ_CP103508:c603953-603489 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT255216 WP_001296435.1 NZ_CP103508:c604288-603953 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References