255140

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4063251..4063945 Replicon chromosome
Accession NZ_CP103479
Organism Escherichia coli strain 4621

Toxin (Protein)


Gene name yafO Uniprot ID V0YKG6
Locus tag M5T46_RS19395 Protein ID WP_001263485.1
Coordinates 4063251..4063649 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5T46_RS19400 Protein ID WP_000554758.1
Coordinates 4063652..4063945 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T46_RS19365 (4058385) 4058385..4059842 + 1458 WP_001293011.1 cytosol nonspecific dipeptidase -
M5T46_RS19370 (4059851) 4059851..4060132 + 282 WP_000106438.1 hypothetical protein -
M5T46_RS19375 (4060149) 4060149..4060658 - 510 WP_000469793.1 metal-dependent hydrolase -
M5T46_RS19380 (4060720) 4060720..4061334 - 615 WP_000602129.1 peptide chain release factor H -
M5T46_RS19385 (4061331) 4061331..4062470 - 1140 WP_000521554.1 RNA ligase RtcB family protein -
M5T46_RS19390 (4062789) 4062789..4063241 - 453 WP_001059900.1 GNAT family N-acetyltransferase -
M5T46_RS19395 (4063251) 4063251..4063649 - 399 WP_001263485.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T46_RS19400 (4063652) 4063652..4063945 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T46_RS19405 (4063997) 4063997..4065052 - 1056 WP_001226177.1 DNA polymerase IV -
M5T46_RS19410 (4065123) 4065123..4065908 - 786 WP_000207541.1 putative lateral flagellar export/assembly protein LafU -
M5T46_RS19415 (4065880) 4065880..4067592 + 1713 Protein_3805 flagellar biosynthesis protein FlhA -
M5T46_RS19420 (4067690) 4067690..4068439 - 750 WP_000093945.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15418.84 Da        Isoelectric Point: 7.3840

>T255140 WP_001263485.1 NZ_CP103479:c4063649-4063251 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255140 WP_000554758.1 NZ_CP103479:c4063945-4063652 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YKG6

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References