Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 700143..700942 Replicon chromosome
Accession NZ_CP103479
Organism Escherichia coli strain 4621

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag M5T46_RS03420 Protein ID WP_000347273.1
Coordinates 700143..700607 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag M5T46_RS03425 Protein ID WP_001309780.1
Coordinates 700607..700942 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T46_RS03390 (695144) 695144..695578 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T46_RS03395 (695596) 695596..696474 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T46_RS03400 (696464) 696464..697243 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T46_RS03405 (697254) 697254..697727 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T46_RS03410 (697750) 697750..699030 - 1281 WP_000681959.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T46_RS03415 (699279) 699279..700088 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
M5T46_RS03420 (700143) 700143..700607 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T46_RS03425 (700607) 700607..700942 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T46_RS03430 (701091) 701091..702662 - 1572 WP_181261139.1 galactarate dehydratase -
M5T46_RS03435 (703037) 703037..704371 + 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
M5T46_RS03440 (704387) 704387..705157 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T255126 WP_000347273.1 NZ_CP103479:c700607-700143 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT255126 WP_001309780.1 NZ_CP103479:c700942-700607 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References