Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4946051..4946653 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5S50_RS23975 Protein ID WP_000897305.1
Coordinates 4946342..4946653 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S50_RS23970 Protein ID WP_000356397.1
Coordinates 4946051..4946341 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS23950 (4942553) 4942553..4943455 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5S50_RS23955 (4943452) 4943452..4944087 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5S50_RS23960 (4944084) 4944084..4945013 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
M5S50_RS23965 (4945228) 4945228..4945446 - 219 WP_001298592.1 CopG family transcriptional regulator -
M5S50_RS23970 (4946051) 4946051..4946341 - 291 WP_000356397.1 NadS family protein Antitoxin
M5S50_RS23975 (4946342) 4946342..4946653 - 312 WP_000897305.1 hypothetical protein Toxin
M5S50_RS23980 (4946882) 4946882..4947790 + 909 WP_001318161.1 alpha/beta hydrolase -
M5S50_RS23985 (4947854) 4947854..4948795 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5S50_RS23990 (4948840) 4948840..4949277 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
M5S50_RS23995 (4949274) 4949274..4950146 - 873 WP_000920762.1 virulence factor BrkB family protein -
M5S50_RS24000 (4950140) 4950140..4950739 - 600 WP_001314324.1 glucose-1-phosphatase -
M5S50_RS24005 (4950838) 4950838..4951623 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T255117 WP_000897305.1 NZ_CP103468:c4946653-4946342 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT255117 WP_000356397.1 NZ_CP103468:c4946341-4946051 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References