Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4008176..4008870 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M5S50_RS19455 Protein ID WP_001263491.1
Coordinates 4008176..4008574 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag M5S50_RS19460 Protein ID WP_000554755.1
Coordinates 4008577..4008870 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS19425 (4003438) 4003438..4004895 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
M5S50_RS19430 (4004904) 4004904..4005185 + 282 WP_022645226.1 hypothetical protein -
M5S50_RS19435 (4005202) 4005202..4005711 - 510 WP_001361775.1 metal-dependent hydrolase -
M5S50_RS19440 (4005773) 4005773..4006387 - 615 WP_022645225.1 peptide chain release factor H -
M5S50_RS19445 (4006384) 4006384..4007523 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
M5S50_RS19450 (4007714) 4007714..4008166 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
M5S50_RS19455 (4008176) 4008176..4008574 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5S50_RS19460 (4008577) 4008577..4008870 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5S50_RS19465 (4008922) 4008922..4009977 - 1056 WP_022645222.1 DNA polymerase IV -
M5S50_RS19470 (4010048) 4010048..4010833 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
M5S50_RS19475 (4010805) 4010805..4012517 + 1713 Protein_3819 flagellar biosynthesis protein FlhA -
M5S50_RS19480 (4012615) 4012615..4013388 - 774 WP_022645219.1 C40 family peptidase -
M5S50_RS19485 (4013574) 4013574..4013834 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T255111 WP_001263491.1 NZ_CP103468:c4008574-4008176 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT255111 WP_000554755.1 NZ_CP103468:c4008870-4008577 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References