Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3768845..3769524 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag M5S50_RS18220 Protein ID WP_000057541.1
Coordinates 3769222..3769524 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5S50_RS18215 Protein ID WP_000806442.1
Coordinates 3768845..3769186 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS18205 (3765088) 3765088..3766020 - 933 WP_022645289.1 glutaminase A -
M5S50_RS18210 (3766283) 3766283..3768787 + 2505 WP_038431953.1 copper-exporting P-type ATPase CopA -
M5S50_RS18215 (3768845) 3768845..3769186 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5S50_RS18220 (3769222) 3769222..3769524 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S50_RS18225 (3769657) 3769657..3770451 + 795 WP_022645287.1 TraB/GumN family protein -
M5S50_RS18230 (3770655) 3770655..3771134 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5S50_RS18235 (3771171) 3771171..3772823 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
M5S50_RS18240 (3773041) 3773041..3774261 + 1221 WP_022645286.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T255109 WP_000057541.1 NZ_CP103468:c3769524-3769222 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT255109 WP_000806442.1 NZ_CP103468:c3769186-3768845 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References