Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 632394..633193 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag M5S50_RS03010 Protein ID WP_000347273.1
Coordinates 632394..632858 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag M5S50_RS03015 Protein ID WP_001308975.1
Coordinates 632858..633193 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS02980 (627395) 627395..627829 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S50_RS02985 (627847) 627847..628725 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S50_RS02990 (628715) 628715..629494 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S50_RS02995 (629505) 629505..629978 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S50_RS03000 (630001) 630001..631281 - 1281 WP_023908831.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S50_RS03005 (631530) 631530..632339 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5S50_RS03010 (632394) 632394..632858 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S50_RS03015 (632858) 632858..633193 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S50_RS03020 (633342) 633342..634913 - 1572 WP_023908830.1 galactarate dehydratase -
M5S50_RS03025 (635288) 635288..636622 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5S50_RS03030 (636638) 636638..637408 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T255098 WP_000347273.1 NZ_CP103468:c632858-632394 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT255098 WP_001308975.1 NZ_CP103468:c633193-632858 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References