Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 184977..186295 Replicon chromosome
Accession NZ_CP103465
Organism Escherichia coli O25b:H4-ST131 strain 5392

Toxin (Protein)


Gene name HipT Uniprot ID F4T5A3
Locus tag M5T42_RS00880 Protein ID WP_001262467.1
Coordinates 185288..186295 (+) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID F4T5A4
Locus tag M5T42_RS00875 Protein ID WP_001312177.1
Coordinates 184977..185288 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T42_RS00845 (180264) 180264..180491 - 228 WP_000198577.1 hypothetical protein -
M5T42_RS00850 (180509) 180509..181528 + 1020 WP_000938855.1 dipeptide ABC transporter permease DppB -
M5T42_RS00855 (181538) 181538..182440 + 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
M5T42_RS00860 (182451) 182451..183434 + 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
M5T42_RS00865 (183431) 183431..184444 + 1014 WP_000103572.1 dipeptide ABC transporter ATP-binding subunit DppF -
M5T42_RS00870 (184669) 184669..184992 + 324 WP_000563102.1 helix-turn-helix transcriptional regulator -
M5T42_RS00875 (184977) 184977..185288 + 312 WP_001312177.1 HipA N-terminal domain-containing protein Antitoxin
M5T42_RS00880 (185288) 185288..186295 + 1008 WP_001262467.1 HipA domain-containing protein Toxin
M5T42_RS00885 (186312) 186312..187583 - 1272 WP_001545666.1 amino acid permease -
- (187945) 187945..188010 - 66 NuclAT_27 - -
- (187945) 187945..188010 - 66 NuclAT_27 - -
- (187945) 187945..188010 - 66 NuclAT_27 - -
- (187945) 187945..188010 - 66 NuclAT_27 - -
M5T42_RS00890 (188059) 188059..188166 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
- (188428) 188428..188485 - 58 NuclAT_26 - -
- (188428) 188428..188485 - 58 NuclAT_26 - -
- (188428) 188428..188485 - 58 NuclAT_26 - -
- (188428) 188428..188485 - 58 NuclAT_26 - -
M5T42_RS00895 (188542) 188542..188649 + 108 WP_000170748.1 type I toxin-antitoxin system toxin Ldr family protein -
M5T42_RS00900 (189025) 189025..189132 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
M5T42_RS00905 (189218) 189218..190897 - 1680 Protein_178 cellulose biosynthesis protein BcsG -
M5T42_RS00910 (190894) 190894..191085 - 192 WP_000988308.1 cellulose biosynthesis protein BcsF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38320.60 Da        Isoelectric Point: 5.5965

>T255070 WP_001262467.1 NZ_CP103465:185288-186295 [Escherichia coli O25b:H4-ST131]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQIMD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFHSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11821.47 Da        Isoelectric Point: 6.0609

>AT255070 WP_001312177.1 NZ_CP103465:184977-185288 [Escherichia coli O25b:H4-ST131]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAPDYHGPAISISLPVAQRQFPSEMLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3K2ZTB5

Antitoxin

Source ID Structure
AlphaFold DB A0A3L3HKE2

References