Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 19002..19726 Replicon plasmid unnamed2
Accession NZ_CP103422
Organism Klebsiella variicola strain KV1

Toxin (Protein)


Gene name higB Uniprot ID A0A2J4YLV3
Locus tag NYO12_RS29845 Protein ID WP_023292165.1
Coordinates 19415..19726 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NYO12_RS29840 Protein ID WP_023292164.1
Coordinates 19002..19418 (-) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO12_RS29825 (NYO12_29825) 14436..15005 - 570 WP_259075151.1 fertility inhibition protein FinO -
NYO12_RS29830 (NYO12_29830) 15105..15827 - 723 WP_259075153.1 type-F conjugative transfer system pilin acetylase TraX -
NYO12_RS29835 (NYO12_29835) 15907..18615 - 2709 Protein_15 AAA family ATPase -
NYO12_RS29840 (NYO12_29840) 19002..19418 - 417 WP_023292164.1 helix-turn-helix domain-containing protein Antitoxin
NYO12_RS29845 (NYO12_29845) 19415..19726 - 312 WP_023292165.1 type II toxin-antitoxin system HigB family toxin Toxin
NYO12_RS29850 (NYO12_29850) 19962..21449 - 1488 WP_161267625.1 group II intron reverse transcriptase/maturase -
NYO12_RS29855 (NYO12_29855) 21855..22283 + 429 WP_259075156.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
NYO12_RS29860 (NYO12_29860) 22283..23554 + 1272 WP_259075159.1 Y-family DNA polymerase -
NYO12_RS29865 (NYO12_29865) 23636..24610 - 975 WP_001568034.1 ParB family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..81805 81805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(21-93)

Antitoxin

(5-138)


Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12497.32 Da        Isoelectric Point: 9.9242

>T255045 WP_023292165.1 NZ_CP103422:c19726-19415 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp


Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15462.59 Da        Isoelectric Point: 4.4720

>AT255045 WP_023292164.1 NZ_CP103422:c19418-19002 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLAARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMRQYGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2J4YLV3


Antitoxin

Source ID Structure

References