Detailed information of TA system TA254879

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103927..104353
• Replicon: plasmid unnamed1
• Accession: NZ_CP103296
• Organism: Escherichia coli strain 2e

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: NW892_RS23370
• Protein ID: WP_001323520.1
• Coordinates: 104237..104353 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	103927..104151 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NW892_RS23315 (99303)	99303..100274	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
NW892_RS23320 (100911)	100911..101080	+	170	Protein_109	hypothetical protein	-
NW892_RS23325 (101263)	101263..101343	-	81	Protein_110	hypothetical protein	-
NW892_RS23330 (101413)	101413..101619	+	207	WP_000275856.1	hypothetical protein	-
NW892_RS23335 (101645)	101645..102184	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
NW892_RS23340 (102252)	102252..102485	+	234	WP_000005987.1	DUF905 family protein	-
NW892_RS23345 (102513)	102513..102710	+	198	Protein_114	hypothetical protein	-
NW892_RS23350 (102765)	102765..103199	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
NW892_RS23355 (103196)	103196..103958	+	763	Protein_116	plasmid SOS inhibition protein A	-
NW892_RS23360 (103927)	103927..104115	-	189	WP_001299721.1	hypothetical protein	-
- (103927)	103927..104151	+	225	NuclAT_0	-	Antitoxin
- (103927)	103927..104151	+	225	NuclAT_0	-	Antitoxin
- (103927)	103927..104151	+	225	NuclAT_0	-	Antitoxin
- (103927)	103927..104151	+	225	NuclAT_0	-	Antitoxin
NW892_RS23365 (104137)	104137..104286	+	150	Protein_118	plasmid maintenance protein Mok	-
NW892_RS23370 (104237)	104237..104353	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
NW892_RS23375 (104573)	104573..104803	+	231	WP_071586998.1	hypothetical protein	-
NW892_RS23380 (104801)	104801..104973	-	173	Protein_121	hypothetical protein	-
NW892_RS23385 (105043)	105043..105249	+	207	WP_000547968.1	hypothetical protein	-
NW892_RS23390 (105274)	105274..105561	+	288	WP_000107535.1	hypothetical protein	-
NW892_RS23395 (105679)	105679..106500	+	822	WP_001234426.1	DUF932 domain-containing protein	-
NW892_RS23400 (106797)	106797..107399	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
NW892_RS23405 (107720)	107720..108103	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / aph(3')-Ia / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..108288	108288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T254879 WP_001323520.1 NZ_CP103296:104237-104353 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT254879 NZ_CP103296:103927-104151 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin