Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3941861..3942588 Replicon chromosome
Accession NZ_CP103295
Organism Escherichia coli strain 2e

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NW892_RS18885 Protein ID WP_000547564.1
Coordinates 3942277..3942588 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NW892_RS18880 Protein ID WP_000126294.1
Coordinates 3941861..3942280 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW892_RS18860 (3936911) 3936911..3937438 - 528 WP_001078777.1 electron transport protein HydN -
NW892_RS18865 (3937587) 3937587..3938597 - 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NW892_RS18870 (3938857) 3938857..3940314 + 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NW892_RS18875 (3940323) 3940323..3941747 + 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NW892_RS18880 (3941861) 3941861..3942280 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NW892_RS18885 (3942277) 3942277..3942588 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NW892_RS18890 (3942762) 3942762..3943523 - 762 WP_001026446.1 hypothetical protein -
NW892_RS18895 (3943548) 3943548..3944018 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NW892_RS18900 (3944011) 3944011..3944421 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NW892_RS18905 (3944418) 3944418..3945185 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NW892_RS18910 (3945185) 3945185..3945727 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NW892_RS18915 (3945737) 3945737..3947446 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T254870 WP_000547564.1 NZ_CP103295:c3942588-3942277 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT254870 WP_000126294.1 NZ_CP103295:c3942280-3941861 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References