25485

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3533616..3534286 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MAF_RS16615 Protein ID WP_003899954.1
Coordinates 3533616..3533960 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MAF_RS16620 Protein ID WP_003899955.1
Coordinates 3533957..3534286 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS16585 3528689..3529549 + 861 WP_003416628.1 alpha/beta hydrolase -
MAF_RS16590 3529524..3530039 + 516 WP_187704705.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MAF_RS16595 3530279..3530572 + 294 WP_003416635.1 hypothetical protein -
MAF_RS16600 3530860..3532149 + 1290 WP_003416640.1 ATP-binding protein -
MAF_RS16605 3532496..3532930 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MAF_RS16610 3532933..3533385 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MAF_RS16615 3533616..3533960 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MAF_RS16620 3533957..3534286 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MAF_RS16625 3534824..3535171 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MAF_RS16630 3535168..3535788 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MAF_RS16635 3535948..3537213 - 1266 WP_013988979.1 hypothetical protein -
MAF_RS16640 3537381..3537590 + 210 WP_003416778.1 hypothetical protein -
MAF_RS16650 3537837..3538871 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T25485 WP_003899954.1 NC_015758:3533616-3533960 [Mycobacterium tuberculosis variant africanum GM041182]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T25485 NC_015758:3533616-3533960 [Mycobacterium tuberculosis variant africanum GM041182]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT25485 WP_003899955.1 NC_015758:3533957-3534286 [Mycobacterium tuberculosis variant africanum GM041182]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT25485 NC_015758:3533957-3534286 [Mycobacterium tuberculosis variant africanum GM041182]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
AlphaFold DB G0THF6

References