Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3844476..3845073 Replicon chromosome
Accession NZ_CP102993
Organism Klebsiella pneumoniae strain KPA3

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NW255_RS18850 Protein ID WP_004142563.1
Coordinates 3844756..3845073 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NW255_RS18845 Protein ID WP_004142561.1
Coordinates 3844476..3844763 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW255_RS18815 (3840556) 3840556..3840804 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NW255_RS18820 (3840822) 3840822..3841163 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
NW255_RS18825 (3841194) 3841194..3842309 - 1116 WP_258825645.1 MBL fold metallo-hydrolase -
NW255_RS18830 (3842489) 3842489..3843070 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
NW255_RS18835 (3843070) 3843070..3843438 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NW255_RS18840 (3843558) 3843558..3844211 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NW255_RS18845 (3844476) 3844476..3844763 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NW255_RS18850 (3844756) 3844756..3845073 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NW255_RS18855 (3845258) 3845258..3846301 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NW255_RS18860 (3846971) 3846971..3847837 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NW255_RS18865 (3847946) 3847946..3849373 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T254818 WP_004142563.1 NZ_CP102993:c3845073-3844756 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT254818 WP_004142561.1 NZ_CP102993:c3844763-3844476 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References