Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 97558..98285 | Replicon | plasmid p1 |
| Accession | NZ_CP102988 | ||
| Organism | Klebsiella pneumoniae strain KPA2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | NW253_RS26375 | Protein ID | WP_011251285.1 |
| Coordinates | 97974..98285 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | NW253_RS26370 | Protein ID | WP_011251286.1 |
| Coordinates | 97558..97977 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NW253_RS26350 (NW253_26350) | 92639..95215 | + | 2577 | WP_017901322.1 | nuclease domain-containing protein | - |
| NW253_RS26355 (NW253_26355) | 95468..95698 | + | 231 | WP_001261275.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| NW253_RS26360 (NW253_26360) | 95695..96111 | + | 417 | WP_000754567.1 | type II toxin-antitoxin system VapC family toxin | - |
| NW253_RS26365 (NW253_26365) | 96419..97387 | + | 969 | WP_016151349.1 | IS5 family transposase | - |
| NW253_RS26370 (NW253_26370) | 97558..97977 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| NW253_RS26375 (NW253_26375) | 97974..98285 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| NW253_RS26380 (NW253_26380) | 98490..98927 | - | 438 | Protein_103 | DDE-type integrase/transposase/recombinase | - |
| NW253_RS26385 (NW253_26385) | 99041..99379 | + | 339 | WP_023288266.1 | transposase | - |
| NW253_RS26390 (NW253_26390) | 99376..99723 | + | 348 | WP_032430752.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| NW253_RS26395 (NW253_26395) | 99772..101305 | + | 1534 | Protein_106 | IS66 family transposase | - |
| NW253_RS26400 (NW253_26400) | 101345..101524 | + | 180 | Protein_107 | IS5/IS1182 family transposase | - |
| NW253_RS26405 (NW253_26405) | 102069..103049 | - | 981 | WP_048293850.1 | IS5-like element ISKpn26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | qnrB4 / blaDHA-1 / aph(3')-Ia / mph(A) | - | 1..188688 | 188688 | |
| - | inside | IScluster/Tn | - | - | 96419..103049 | 6630 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T254808 WP_011251285.1 NZ_CP102988:c98285-97974 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT254808 WP_011251286.1 NZ_CP102988:c97977-97558 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|