Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2202253..2203121 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MAF_RS10220 Protein ID WP_010886136.1
Coordinates 2202253..2202630 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MAF_RS10225 Protein ID WP_003409886.1
Coordinates 2202672..2203121 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS10170 2198042..2198494 - 453 WP_003899095.1 lipoprotein -
MAF_RS10175 2198558..2198959 + 402 WP_003409869.1 hypothetical protein -
MAF_RS10180 2198952..2199134 - 183 WP_003409870.1 hypothetical protein -
MAF_RS10185 2199248..2199598 - 351 WP_003910480.1 hypothetical protein -
MAF_RS10190 2199609..2200511 - 903 WP_003409874.1 hypothetical protein -
MAF_RS10195 2200532..2200723 - 192 WP_003409876.1 hypothetical protein -
MAF_RS10200 2200724..2201020 - 297 WP_003409877.1 hypothetical protein -
MAF_RS10205 2201260..2201475 + 216 WP_003409878.1 antitoxin -
MAF_RS10210 2201472..2201783 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MAF_RS22135 2201757..2202278 - 522 WP_003904745.1 hypothetical protein -
MAF_RS10220 2202253..2202630 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MAF_RS10225 2202672..2203121 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MAF_RS10230 2203118..2203663 + 546 WP_003409891.1 SecB-like chaperone -
MAF_RS10235 2203552..2204166 - 615 WP_003901296.1 hypothetical protein -
MAF_RS10240 2204215..2204511 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MAF_RS10245 2204508..2204759 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MAF_RS10250 2204746..2205240 + 495 WP_003899099.1 hypothetical protein -
MAF_RS10255 2205400..2205807 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MAF_RS10260 2205811..2206083 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MAF_RS10265 2206116..2207336 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T25462 WP_010886136.1 NC_015758:2202253-2202630 [Mycobacterium tuberculosis variant africanum GM041182]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T25462 NC_015758:2202253-2202630 [Mycobacterium tuberculosis variant africanum GM041182]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT25462 WP_003409886.1 NC_015758:2202672-2203121 [Mycobacterium tuberculosis variant africanum GM041182]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT25462 NC_015758:2202672-2203121 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References