Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 15226..15953 Replicon plasmid pKA1-1
Accession NZ_CP102894
Organism Klebsiella quasipneumoniae strain KA1

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag NWT75_RS25365 Protein ID WP_011251285.1
Coordinates 15226..15537 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NWT75_RS25370 Protein ID WP_011251286.1
Coordinates 15534..15953 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT75_RS25335 (NWT75_25335) 11183..11620 - 438 WP_071444170.1 hypothetical protein -
NWT75_RS25340 (NWT75_25340) 11664..12632 + 969 WP_258336593.1 IS5-like element IS903B family transposase -
NWT75_RS25345 (NWT75_25345) 12690..13739 - 1050 Protein_14 IS66 family transposase -
NWT75_RS25350 (NWT75_25350) 13788..14135 - 348 WP_094320106.1 IS66 family insertion sequence element accessory protein TnpB -
NWT75_RS25355 (NWT75_25355) 14132..14470 - 339 WP_023288266.1 transposase -
NWT75_RS25360 (NWT75_25360) 14584..15021 + 438 Protein_17 DDE-type integrase/transposase/recombinase -
NWT75_RS25365 (NWT75_25365) 15226..15537 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
NWT75_RS25370 (NWT75_25370) 15534..15953 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
NWT75_RS25375 (NWT75_25375) 16100..17068 + 969 WP_258336594.1 IS5 family transposase -
NWT75_RS25380 (NWT75_25380) 17265..18632 - 1368 WP_258336595.1 formimidoylglutamate deiminase -
NWT75_RS25385 (NWT75_25385) 18735..19496 + 762 WP_017900871.1 histidine utilization repressor -
NWT75_RS25390 (NWT75_25390) 19493..20050 + 558 WP_017900872.1 HutD family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..184774 184774
- inside IScluster/Tn - - 11664..17068 5404


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T254426 WP_011251285.1 NZ_CP102894:15226-15537 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT254426 WP_011251286.1 NZ_CP102894:15534-15953 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References