Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 69387..70114 Replicon plasmid pKA2-1
Accession NZ_CP102870
Organism Klebsiella pneumoniae strain KA2

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag NWT85_RS26140 Protein ID WP_011251285.1
Coordinates 69387..69698 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NWT85_RS26145 Protein ID WP_011251286.1
Coordinates 69695..70114 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT85_RS26115 (NWT85_26115) 66104..66283 - 180 Protein_71 IS5/IS1182 family transposase -
NWT85_RS26120 (NWT85_26120) 66323..67861 - 1539 WP_017901237.1 IS66 family transposase -
NWT85_RS26125 (NWT85_26125) 67910..68257 - 348 WP_032430752.1 IS66 family insertion sequence element accessory protein TnpB -
NWT85_RS26130 (NWT85_26130) 68254..68631 - 378 WP_004118218.1 transposase -
NWT85_RS26135 (NWT85_26135) 68745..69182 + 438 Protein_75 DDE-type integrase/transposase/recombinase -
NWT85_RS26140 (NWT85_26140) 69387..69698 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
NWT85_RS26145 (NWT85_26145) 69695..70114 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
NWT85_RS26150 (NWT85_26150) 70184..70597 + 414 WP_017901337.1 hypothetical protein -
NWT85_RS26155 (NWT85_26155) 70643..71611 - 969 WP_258335056.1 IS5-like element IS903B family transposase -
NWT85_RS26160 (NWT85_26160) 71715..71981 + 267 WP_017900868.1 hypothetical protein -
NWT85_RS26165 (NWT85_26165) 72095..72630 - 536 Protein_81 transposase -
NWT85_RS26170 (NWT85_26170) 73155..74522 - 1368 WP_017900870.1 formimidoylglutamate deiminase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / tet(A) / mph(E) / msr(E) / armA / blaDHA-1 / qnrB4 / blaTEM-1B / aac(3)-IId - 1..257713 257713
- inside IScluster/Tn - - 63801..72489 8688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T254342 WP_011251285.1 NZ_CP102870:69387-69698 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT254342 WP_011251286.1 NZ_CP102870:69695-70114 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References