Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3662295..3662836 Replicon chromosome
Accession NZ_CP102853
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag NWH85_RS17945 Protein ID WP_000615976.1
Coordinates 3662558..3662836 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag NWH85_RS17940 Protein ID WP_000729704.1
Coordinates 3662295..3662555 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS17915 (3658078) 3658078..3658863 - 786 WP_191631332.1 putative lateral flagellar export/assembly protein LafU -
NWH85_RS17920 (3658835) 3658835..3660547 + 1713 Protein_3506 flagellar biosynthesis protein FlhA -
NWH85_RS17925 (3660652) 3660652..3660930 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
NWH85_RS17930 (3660923) 3660923..3661279 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
NWH85_RS17935 (3661336) 3661336..3662109 - 774 WP_000543895.1 C40 family peptidase -
NWH85_RS17940 (3662295) 3662295..3662555 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NWH85_RS17945 (3662558) 3662558..3662836 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NWH85_RS17950 (3662992) 3662992..3663732 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NWH85_RS17955 (3663703) 3663703..3664470 - 768 WP_000333380.1 class II glutamine amidotransferase -
NWH85_RS17960 (3664676) 3664676..3665254 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / vgrG/tssI / tssM / hcp1/tssD1 3660923..3685908 24985


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T254303 WP_000615976.1 NZ_CP102853:3662558-3662836 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT254303 WP_000729704.1 NZ_CP102853:3662295-3662555 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References