Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 593394..594193 Replicon chromosome
Accession NZ_CP102853
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag NWH85_RS02895 Protein ID WP_000347266.1
Coordinates 593394..593858 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NWH85_RS02900 Protein ID WP_001307405.1
Coordinates 593858..594193 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS02865 (588395) 588395..588829 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NWH85_RS02870 (588847) 588847..589725 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
NWH85_RS02875 (589715) 589715..590494 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NWH85_RS02880 (590505) 590505..590978 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NWH85_RS02885 (591001) 591001..592281 - 1281 WP_258334515.1 tagatose-bisphosphate aldolase subunit KbaZ -
NWH85_RS02890 (592530) 592530..593339 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NWH85_RS02895 (593394) 593394..593858 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NWH85_RS02900 (593858) 593858..594193 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NWH85_RS02905 (594342) 594342..595913 - 1572 WP_001273741.1 galactarate dehydratase -
NWH85_RS02910 (596288) 596288..597622 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NWH85_RS02915 (597638) 597638..598408 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 593394..605068 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T254290 WP_000347266.1 NZ_CP102853:c593858-593394 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT254290 WP_001307405.1 NZ_CP102853:c594193-593858 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References