Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1381953..1382546 Replicon chromosome
Accession NC_015726
Organism Cupriavidus necator N-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CNE_RS06685 Protein ID WP_041228359.1
Coordinates 1382268..1382546 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CNE_RS06680 Protein ID WP_013956365.1
Coordinates 1381953..1382255 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNE_RS06635 1377384..1377773 + 390 WP_013956354.1 hypothetical protein -
CNE_RS06640 1377770..1378186 + 417 WP_013956355.1 hypothetical protein -
CNE_RS06645 1378297..1378602 + 306 WP_013956357.1 hypothetical protein -
CNE_RS41510 1378967..1379137 + 171 WP_013956358.1 hypothetical protein -
CNE_RS06655 1379418..1380029 + 612 WP_148271569.1 hypothetical protein -
CNE_RS40960 1380259..1380489 - 231 WP_148271570.1 hypothetical protein -
CNE_RS06665 1380624..1380878 - 255 WP_013956362.1 hypothetical protein -
CNE_RS06670 1380903..1381193 - 291 WP_041227864.1 DUF2471 family protein -
CNE_RS06675 1381549..1381860 + 312 WP_013956364.1 hypothetical protein -
CNE_RS06680 1381953..1382255 - 303 WP_013956365.1 HigA family addiction module antidote protein Antitoxin
CNE_RS06685 1382268..1382546 - 279 WP_041228359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNE_RS06690 1382861..1383367 - 507 WP_013956367.1 cupin domain-containing protein -
CNE_RS06695 1383411..1383671 - 261 WP_035817461.1 hypothetical protein -
CNE_RS06700 1383898..1384284 + 387 WP_013956369.1 hypothetical protein -
CNE_RS06705 1384325..1384558 + 234 WP_035817458.1 hypothetical protein -
CNE_RS06710 1384650..1384838 + 189 WP_018310329.1 hypothetical protein -
CNE_RS06715 1384939..1385298 + 360 WP_013956372.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(24-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-66)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10408.91 Da        Isoelectric Point: 8.3165

>T25418 WP_041228359.1 NC_015726:c1382546-1382268 [Cupriavidus necator N-1]
MITSFNCRDTALLFAGTRIARFVNIESVAMRKLQQLHAAGDLSFLRVPPGNRLEALQGHRAGQHSIRINDQWRICFCFAN
GHATDVEIVDYH

Download         Length: 279 bp

>T25418 NC_015726:c1382546-1382268 [Cupriavidus necator N-1]
ATGATCACTTCGTTCAACTGCCGCGACACGGCACTGCTCTTCGCTGGCACGCGGATCGCCCGGTTCGTCAACATAGAATC
TGTTGCCATGCGCAAGCTCCAGCAGTTGCACGCGGCCGGCGACCTCAGCTTTCTGCGAGTCCCGCCGGGTAACAGGCTGG
AAGCGCTGCAAGGGCACCGGGCTGGTCAGCACAGCATCCGTATCAACGACCAATGGCGAATCTGCTTTTGCTTCGCCAAT
GGCCATGCAACCGACGTCGAGATCGTCGACTACCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11067.78 Da        Isoelectric Point: 6.4670

>AT25418 WP_013956365.1 NC_015726:c1382255-1381953 [Cupriavidus necator N-1]
MAREVPLATPGEILQLDWLDPLGISQYALAKAIDVPPRRINEIVKGGRAITADTAVRLGAFFGTDPQSWMNLQTHFDTEM
AKEKIGEKKLESIRQHAVLA

Download         Length: 303 bp

>AT25418 NC_015726:c1382255-1381953 [Cupriavidus necator N-1]
ATGGCACGCGAAGTACCCCTGGCCACTCCGGGAGAAATCCTTCAACTGGACTGGCTCGACCCGCTTGGCATTTCTCAGTA
CGCCCTGGCCAAGGCCATCGACGTACCGCCTCGCCGTATCAATGAAATTGTGAAGGGTGGCCGCGCCATAACGGCGGACA
CCGCTGTACGCCTTGGTGCGTTCTTCGGCACCGATCCCCAGAGTTGGATGAACCTGCAGACGCACTTCGACACCGAAATG
GCAAAGGAGAAGATCGGCGAGAAGAAGCTGGAGTCGATCAGGCAGCATGCCGTGCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References