Detailed information of TA system TA025414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1695515..1696140
• Replicon: chromosome
• Accession: NC_015723
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CNE_RS25720
• Protein ID: WP_041228659.1
• Coordinates: 1695515..1695793 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNE_RS25725
• Protein ID: WP_013953221.1
• Coordinates: 1695805..1696140 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS25720	1695515..1695793	+	279	WP_041228659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNE_RS25725	1695805..1696140	+	336	WP_013953221.1	HigA family addiction module antidote protein	Antitoxin
CNE_RS25730	1696190..1697560	-	1371	WP_013953222.1	serine hydrolase	-
CNE_RS25735	1697607..1698086	-	480	WP_013953223.1	MarR family transcriptional regulator	-
CNE_RS25740	1698265..1698861	-	597	WP_013953224.1	GTP cyclohydrolase II	-
CNE_RS25745	1699129..1699659	+	531	WP_013953225.1	hypothetical protein	-
CNE_RS25750	1699660..1700130	-	471	WP_010814564.1	Lrp/AsnC family transcriptional regulator	-
CNE_RS25755	1700283..1700624	+	342	WP_041228660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-94)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10589.19 Da
• Isoelectric Point: 9.9483

>T25414 WP_041228659.1 NC_015723:1695515-1695793 [Cupriavidus necator N-1]
MIQSFRCSDTAELFAGRCVPRFGNIRAVAERKLRLLQSASTLEFPRSPPGNRLQLLAGDRKGRYSIRINEQWRICFIWTH
DGPAAVEIIDYH

Nucleotide

• Length: 279 bp

>T25414 NC_015723:1695515-1695793 [Cupriavidus necator N-1]
ATGATCCAGTCATTCAGATGTTCCGACACCGCTGAACTGTTCGCGGGCCGCTGCGTACCACGCTTCGGCAATATCCGCGC
CGTGGCCGAACGCAAGCTGCGGCTGCTGCAGAGTGCGTCGACGCTGGAGTTCCCGCGCTCCCCGCCGGGCAATCGCCTGC
AGTTACTGGCGGGCGACCGCAAAGGCCGCTACAGCATCCGCATCAACGAACAGTGGCGAATCTGCTTCATCTGGACCCAC
GACGGCCCGGCGGCCGTTGAGATCATCGACTATCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12249.09 Da
• Isoelectric Point: 9.8246

>AT25414 WP_013953221.1 NC_015723:1695805-1696140 [Cupriavidus necator N-1]
MVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYG
LKVAQRDHGERIMAEVAPRNAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT25414 NC_015723:1695805-1696140 [Cupriavidus necator N-1]
ATGGTTCGCTTCAAGGACGGCATGCGTCCCATCCACCCGGGCGAAATCCTGCGCGAGGATTTCCTGGTACCGCTCAACAT
GAGCGCCAACGCCCTGGCCCTCGCGCTGCGCGTGACCGCGGCGCGCATCAACGACATCGTCCGCGAGCAACGTGGCATCA
CGCCGGACACCGCCCTGCGCCTGGCGCGCTATTTTGGCGGCGATGCGCAGTCGTGGTTGAACTTGCAGCTGGACTATGGC
CTGAAGGTCGCGCAGCGCGACCACGGCGAGCGGATCATGGCGGAAGTGGCGCCGCGCAATGCGGCGGCGGCCGGTCAGCC
GGCTGCGGAAAAGTAG