Detailed information of TA system TA025408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3328643..3329289
• Replicon: chromosome
• Accession: NC_015684
• Organism: Oligotropha carboxidovorans OM5

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B6JDM4
• Locus tag: OCA5_RS15720
• Protein ID: WP_012561984.1
• Coordinates: 3328643..3328924 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCA5_RS15725
• Protein ID: WP_012561983.1
• Coordinates: 3328933..3329289 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCA5_RS15680	3323660..3324244	-	585	WP_013913374.1	hypothetical protein	-
OCA5_RS15685	3324433..3324651	+	219	WP_012561990.1	hypothetical protein	-
OCA5_RS15690	3324756..3325475	+	720	WP_013913375.1	DUF3489 domain-containing protein	-
OCA5_RS15695	3325601..3325831	+	231	WP_012561988.1	hexameric tyrosine-coordinated heme protein	-
OCA5_RS18940	3325849..3326925	-	1077	WP_012561987.1	DNA cytosine methyltransferase	-
OCA5_RS15715	3326927..3328294	-	1368	WP_012561986.1	site-specific DNA-methyltransferase	-
OCA5_RS15720	3328643..3328924	+	282	WP_012561984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCA5_RS15725	3328933..3329289	+	357	WP_012561983.1	HigA family addiction module antidote protein	Antitoxin
OCA5_RS15730	3329687..3330238	-	552	WP_012561981.1	hypothetical protein	-
OCA5_RS15735	3330235..3330450	-	216	WP_012561980.1	hypothetical protein	-
OCA5_RS15740	3330447..3330764	-	318	WP_013913377.1	hypothetical protein	-
OCA5_RS15745	3330761..3331297	-	537	WP_012561978.1	hypothetical protein	-
OCA5_RS15750	3331630..3331878	-	249	WP_012561977.1	hypothetical protein	-
OCA5_RS15755	3331914..3332177	-	264	WP_013913378.1	SHOCT domain-containing protein	-
OCA5_RS15760	3332198..3332581	-	384	WP_012561975.1	DUF302 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3290883..3362189	71306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (25-111)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10592.14 Da
• Isoelectric Point: 10.1526

>T25408 WP_012561984.1 NC_015684:3328643-3328924 [Afipia carboxidovorans OM5]
VIKSFGDKRTAAIFAGYAVRGLPPPIQERARAKLLVIDAAKQLDDLRQPPGNRLEALAGHRKGQHSIRINDQWRICFVWR
NGETFDVEIVDYH

Nucleotide

• Length: 282 bp

>T25408 NC_015684:3328643-3328924 [Afipia carboxidovorans OM5]
GTGATCAAGAGCTTCGGCGATAAACGCACCGCCGCAATCTTCGCAGGCTACGCCGTGCGCGGTTTGCCGCCGCCGATTCA
AGAGCGGGCTCGGGCGAAGCTGTTGGTGATCGACGCAGCAAAGCAACTCGACGATCTGCGCCAGCCGCCAGGCAACAGAC
TGGAGGCGTTGGCGGGTCACCGTAAGGGCCAGCACAGTATCCGCATCAACGATCAATGGCGGATATGCTTTGTGTGGCGG
AATGGCGAAACCTTCGATGTCGAAATTGTCGACTATCATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13565.63 Da
• Isoelectric Point: 10.5936

>AT25408 WP_012561983.1 NC_015684:3328933-3329289 [Afipia carboxidovorans OM5]
MMSIKRENIDKGEIDFADVRSGRRLPPIHPGTILRDEFLTPMQISVYELASAIKTPRSRINDIVLGRRTITTDTALRLGR
YLGTSPEFWINLQARYDLDVANRTVRRKIEQEITPRAA

Nucleotide

• Length: 357 bp

>AT25408 NC_015684:3328933-3329289 [Afipia carboxidovorans OM5]
ATGATGAGCATCAAGCGTGAAAACATCGACAAGGGAGAGATCGACTTCGCCGACGTGCGTTCCGGGCGACGGCTGCCGCC
AATCCATCCGGGCACGATCCTCCGCGATGAGTTCCTGACCCCTATGCAGATCAGCGTCTATGAGCTTGCCAGCGCAATCA
AGACGCCACGTTCGCGGATCAACGACATCGTACTGGGGCGGCGCACGATTACCACCGATACCGCACTACGGCTCGGACGC
TATCTTGGAACCTCGCCGGAGTTCTGGATCAATCTGCAGGCGCGCTACGATCTCGATGTCGCCAATCGCACCGTTCGCCG
TAAGATCGAACAGGAGATCACGCCACGTGCAGCTTGA

Structures

Toxin

• AlphaFold DB: B6JDM4