Detailed information of TA system TA025407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2770128..2770719
• Replicon: chromosome
• Accession: NC_015684
• Organism: Oligotropha carboxidovorans OM5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B6JBF6
• Locus tag: OCA5_RS13180
• Protein ID: WP_012562526.1
• Coordinates: 2770128..2770409 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCA5_RS13185
• Protein ID: WP_012562525.1
• Coordinates: 2770420..2770719 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCA5_RS13160	2765414..2767222	-	1809	WP_012562530.1	flagellar hook-basal body complex protein	-
OCA5_RS13165	2767402..2767896	-	495	WP_012562529.1	peptide-methionine (R)-S-oxide reductase MsrB	-
OCA5_RS13170	2767989..2769392	-	1404	WP_012562528.1	FAD-binding oxidoreductase	-
OCA5_RS13175	2769508..2770038	+	531	WP_012562527.1	histidine phosphatase family protein	-
OCA5_RS13180	2770128..2770409	+	282	WP_012562526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCA5_RS13185	2770420..2770719	+	300	WP_012562525.1	HigA family addiction module antidote protein	Antitoxin
OCA5_RS13190	2770861..2771304	+	444	WP_041559641.1	hypothetical protein	-
OCA5_RS13195	2771344..2771850	-	507	WP_013913263.1	ferritin-like domain-containing protein	-
OCA5_RS13200	2772256..2773314	+	1059	WP_012562521.1	flippase-like domain-containing protein	-
OCA5_RS13205	2773365..2775338	+	1974	WP_012562520.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10569.07 Da
• Isoelectric Point: 10.2043

>T25407 WP_012562526.1 NC_015684:2770128-2770409 [Afipia carboxidovorans OM5]
VIKSFKDDLTEAAFNGKARKGFPAQLLKVARRKLAMVNAATSLDDLRSPPNNRLEALTRERAGQHSIRVNDQFRICFIWT
KDGAEQVELVDYH

Nucleotide

• Length: 282 bp

>T25407 NC_015684:2770128-2770409 [Afipia carboxidovorans OM5]
GTGATCAAGAGCTTCAAGGACGATCTGACTGAAGCAGCCTTCAACGGGAAGGCACGCAAAGGTTTTCCGGCTCAGTTACT
CAAAGTTGCCCGCCGCAAACTGGCGATGGTGAATGCGGCTACATCCCTGGATGATCTGCGTTCGCCGCCCAACAACCGAC
TTGAAGCTCTCACCAGGGAGCGAGCGGGCCAGCATTCAATTCGGGTGAACGACCAGTTTCGCATCTGCTTCATATGGACG
AAGGATGGAGCTGAACAAGTCGAGCTTGTCGATTATCATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10856.59 Da
• Isoelectric Point: 9.0146

>AT25407 WP_012562525.1 NC_015684:2770420-2770719 [Afipia carboxidovorans OM5]
MAKTLPPLHPGEVLREEYLEPLNLSPGKLAKACGIPRTRIERIASERFGITADTALRLGKALGTSAQFWLNLQNDYDLRV
AKVAIAKDLEKVTSVQEAA

Nucleotide

• Length: 300 bp

>AT25407 NC_015684:2770420-2770719 [Afipia carboxidovorans OM5]
ATGGCCAAGACACTGCCGCCACTGCACCCCGGGGAAGTTCTTCGGGAAGAATATCTGGAGCCGCTGAATCTCTCGCCCGG
CAAACTTGCCAAGGCCTGTGGCATTCCCCGTACGCGCATCGAGCGTATCGCCAGCGAGCGTTTCGGCATTACCGCAGACA
CTGCTCTACGACTCGGCAAGGCGCTCGGTACTTCCGCCCAGTTCTGGCTCAACCTGCAGAATGACTACGATCTGCGGGTG
GCAAAAGTGGCAATCGCGAAGGATCTGGAAAAGGTCACCTCCGTTCAAGAGGCCGCCTAG

Structures

Toxin

• AlphaFold DB: B6JBF6