Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4425148..4425750 Replicon chromosome
Accession NZ_CP102679
Organism Escherichia coli strain XH989

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NU949_RS23760 Protein ID WP_000897305.1
Coordinates 4425439..4425750 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NU949_RS23755 Protein ID WP_000356397.1
Coordinates 4425148..4425438 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NU949_RS23730 (4421093) 4421093..4421995 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
NU949_RS23735 (4421992) 4421992..4422627 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NU949_RS23740 (4422624) 4422624..4423553 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
NU949_RS23745 (4423883) 4423883..4424125 - 243 WP_001087409.1 protein YiiF -
NU949_RS23750 (4424344) 4424344..4424562 - 219 WP_001295676.1 CopG family transcriptional regulator -
NU949_RS23755 (4425148) 4425148..4425438 - 291 WP_000356397.1 NadS family protein Antitoxin
NU949_RS23760 (4425439) 4425439..4425750 - 312 WP_000897305.1 hypothetical protein Toxin
NU949_RS23765 (4425979) 4425979..4426887 + 909 WP_001162704.1 alpha/beta hydrolase -
NU949_RS23770 (4426951) 4426951..4427892 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NU949_RS23775 (4427937) 4427937..4428374 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NU949_RS23780 (4428371) 4428371..4429243 - 873 WP_000920762.1 virulence factor BrkB family protein -
NU949_RS23785 (4429237) 4429237..4429836 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4423883..4433403 9520


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T253992 WP_000897305.1 NZ_CP102679:c4425750-4425439 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT253992 WP_000356397.1 NZ_CP102679:c4425438-4425148 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References