Detailed information of TA system TA025389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1823707..1824283
• Replicon: chromosome
• Accession: NC_015673
• Organism: Corynebacterium resistens DSM 45100

Toxin (Protein)

• Gene name: graT
• Uniprot ID: D7WDU2
• Locus tag: CRES_RS07805
• Protein ID: WP_005290974.1
• Coordinates: 1824002..1824283 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CRES_RS07800
• Protein ID: WP_084767533.1
• Coordinates: 1823707..1824015 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRES_RS07770	1818707..1819066	+	360	WP_012359887.1	metalloregulator ArsR/SmtB family transcription factor	-
CRES_RS07775	1819063..1820955	+	1893	WP_013888870.1	cadmium-translocating P-type ATPase	-
CRES_RS12375	1821564..1821719	-	156	WP_158306471.1	DDE-type integrase/transposase/recombinase	-
CRES_RS12380	1821746..1821907	-	162	WP_013888871.1	hypothetical protein	-
CRES_RS12210	1821993..1822184	-	192	WP_148257578.1	hypothetical protein	-
CRES_RS12410	1822205..1822360	+	156	WP_162467885.1	hypothetical protein	-
CRES_RS07790	1822730..1823106	-	377	Protein_1517	SMI1/KNR4 family protein	-
CRES_RS07795	1823209..1823652	+	444	WP_042380625.1	aminoacyl-tRNA hydrolase	-
CRES_RS07800	1823707..1824015	-	309	WP_084767533.1	HigA family addiction module antidote protein	Antitoxin
CRES_RS07805	1824002..1824283	-	282	WP_005290974.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRES_RS12470	1824519..1824935	+	417	WP_201764182.1	AbiV family abortive infection protein	-
CRES_RS12475	1825046..1825213	+	168	WP_013888876.1	hypothetical protein	-
CRES_RS12480	1825248..1825907	-	660	WP_201764183.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-97)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11051.53 Da
• Isoelectric Point: 9.8469

>T25389 WP_005290974.1 NC_015673:c1824283-1824002 [Corynebacterium resistens DSM 45100]
MIQSFADKDTERLWNRERVRSIDSRIHSVALRKLRQLGYAQTLDELRIPPGNRLEALKGDRRGQFSIRINDQWRICFRWS
AAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T25389 NC_015673:c1824283-1824002 [Corynebacterium resistens DSM 45100]
ATGATCCAGTCGTTCGCTGACAAGGACACTGAGCGTCTATGGAACCGAGAGCGGGTTCGCTCGATCGATTCCCGCATTCA
CTCAGTAGCGCTGCGCAAGCTTCGCCAGCTTGGGTACGCGCAGACTCTTGATGAGTTACGGATTCCCCCGGGAAACCGGC
TCGAGGCGTTGAAAGGTGATCGGCGGGGTCAGTTCAGCATTCGAATCAACGACCAGTGGCGGATCTGTTTTCGGTGGTCC
GCCGCGGGGCCAGAGGAGGTTGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11440.26 Da
• Isoelectric Point: 5.6158

>AT25389 WP_084767533.1 NC_015673:c1824015-1823707 [Corynebacterium resistens DSM 45100]
MTTTDKLPPVHPGEILMEDFLKEMGITQHKLAVSIGVPPRRINEIVHGKRAVTADTALRLAKFFEMSPQFWLGLQTQYDL
DVAEDKILSEIERIQPAQAVSA

Nucleotide

• Length: 309 bp

>AT25389 NC_015673:c1824015-1823707 [Corynebacterium resistens DSM 45100]
TTGACTACCACTGACAAGCTCCCTCCGGTTCACCCCGGTGAGATCCTCATGGAGGACTTTCTCAAGGAAATGGGGATCAC
CCAGCACAAGCTCGCCGTCTCCATCGGCGTTCCGCCCCGCCGGATCAACGAAATTGTTCACGGTAAGCGCGCTGTAACCG
CTGACACGGCCCTTCGTCTAGCGAAGTTTTTCGAGATGAGCCCCCAGTTCTGGCTCGGGTTGCAGACCCAGTACGACTTG
GATGTGGCAGAAGACAAAATCCTCTCGGAAATTGAGCGGATTCAACCGGCCCAAGCTGTCTCAGCGTAG

Structures

Toxin

• AlphaFold DB: A0A1F1ZH13