253755

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4466355..4467082 Replicon chromosome
Accession NZ_CP102488
Organism Escherichia coli O15:H18 strain ST69

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NUI00_RS21755 Protein ID WP_000550189.1
Coordinates 4466355..4466669 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag NUI00_RS21760 Protein ID WP_000560253.1
Coordinates 4466666..4467082 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUI00_RS21735 (4462521) 4462521..4463507 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
NUI00_RS21740 (4463586) 4463586..4464269 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
NUI00_RS21745 (4464346) 4464346..4464849 - 504 WP_001309773.1 M48 family metallopeptidase -
NUI00_RS21750 (4464934) 4464934..4466070 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NUI00_RS21755 (4466355) 4466355..4466669 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NUI00_RS21760 (4466666) 4466666..4467082 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NUI00_RS21765 (4467127) 4467127..4469145 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NUI00_RS21770 (4469316) 4469316..4471667 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T253755 WP_000550189.1 NZ_CP102488:4466355-4466669 [Escherichia coli O15:H18]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT253755 WP_000560253.1 NZ_CP102488:4466666-4467082 [Escherichia coli O15:H18]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References