Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1120574..1121136 Replicon chromosome
Accession NZ_CP102488
Organism Escherichia coli O15:H18 strain ST69

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag NUI00_RS05335 Protein ID WP_000605676.1
Coordinates 1120574..1120852 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag NUI00_RS05340 Protein ID WP_000781370.1
Coordinates 1120852..1121136 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUI00_RS05310 (1116160) 1116160..1116591 - 432 WP_000152305.1 peroxiredoxin OsmC -
NUI00_RS05315 (1116936) 1116936..1117151 + 216 WP_000495766.1 biofilm-dependent modulation protein -
NUI00_RS05320 (1117253) 1117253..1117390 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
NUI00_RS05325 (1117547) 1117547..1119244 + 1698 WP_000433464.1 malate dehydrogenase -
NUI00_RS05330 (1119378) 1119378..1120388 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
NUI00_RS05335 (1120574) 1120574..1120852 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NUI00_RS05340 (1120852) 1120852..1121136 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
NUI00_RS05345 (1121364) 1121364..1122017 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
NUI00_RS05350 (1122010) 1122010..1122894 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
NUI00_RS05355 (1122907) 1122907..1125954 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T253742 WP_000605676.1 NZ_CP102488:1120574-1120852 [Escherichia coli O15:H18]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT253742 WP_000781370.1 NZ_CP102488:1120852-1121136 [Escherichia coli O15:H18]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References