Detailed information of TA system TA025364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 172087..172793
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F8B1V8
• Locus tag: FSYMDG_RS00690
• Protein ID: WP_013871710.1
• Coordinates: 172087..172482 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FSYMDG_RS00695
• Protein ID: WP_013871711.1
• Coordinates: 172479..172793 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS00680	168649..169914	+	1266	WP_013871707.1	LuxR family transcriptional regulator	-
FSYMDG_RS25540	170306..170446	+	141	WP_013871708.1	hypothetical protein	-
FSYMDG_RS00685	170637..171899	-	1263	WP_013872077.1	IS4 family transposase	-
FSYMDG_RS00690	172087..172482	+	396	WP_013871710.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSYMDG_RS00695	172479..172793	+	315	WP_013871711.1	helix-turn-helix transcriptional regulator	Antitoxin
FSYMDG_RS23680	173496..173963	+	468	WP_157490952.1	macro domain-containing protein	-
FSYMDG_RS00700	173990..175425	-	1436	Protein_129	IS66 family transposase	-
FSYMDG_RS23685	175561..176118	+	558	WP_157490953.1	toll/interleukin-1 receptor domain-containing protein	-
FSYMDG_RS00705	176143..177231	+	1089	WP_013871712.1	IS630 family transposase	-
FSYMDG_RS23690	177212..177769	-	558	WP_157490953.1	toll/interleukin-1 receptor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	170637..177231	6594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-117)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14589.77 Da
• Isoelectric Point: 10.5110

>T25364 WP_013871710.1 NC_015656:172087-172482 [Candidatus Frankia datiscae]
MSAEPPHRWAIKVAPEVREWLRALRQTDPGAYRSVNVAIDMLAEIGPGLGRPLVDTLQGSTVKNLKELRPRSGRDVAIQV
IFVFDPWSQAVLLVAGNKAGDWSRWYRTAIPAAEVAYGGWLAVERKRREGS

Nucleotide

• Length: 396 bp

>T25364 NC_015656:172087-172482 [Candidatus Frankia datiscae]
ATGTCGGCGGAGCCGCCTCATCGGTGGGCGATCAAGGTCGCGCCCGAGGTACGGGAGTGGCTGCGTGCGTTGCGCCAGAC
AGATCCGGGCGCCTACCGGTCGGTCAACGTGGCGATCGACATGCTCGCCGAGATCGGGCCTGGGCTCGGGCGTCCGCTGG
TCGACACGCTGCAGGGGTCGACAGTCAAGAATCTCAAGGAGCTGCGTCCACGGTCGGGCCGTGATGTTGCGATACAGGTG
ATCTTTGTCTTCGACCCCTGGTCGCAGGCGGTACTGCTGGTCGCCGGCAACAAGGCTGGCGACTGGTCGCGGTGGTACAG
GACAGCGATCCCCGCGGCCGAGGTCGCGTATGGCGGATGGCTGGCCGTGGAGAGGAAGCGGCGGGAGGGCTCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11769.10 Da
• Isoelectric Point: 4.7424

>AT25364 WP_013871711.1 NC_015656:172479-172793 [Candidatus Frankia datiscae]
MTEFHDWDDIRAELHDGDGEALAAERARTEAWVSAFHLAEERRRLGLTQRQVAELMGVSPGRVSQIENGDLDANEVATLS
RYARALGARMRIIFDYGNDLRQIV

Nucleotide

• Length: 315 bp

>AT25364 NC_015656:172479-172793 [Candidatus Frankia datiscae]
ATGACCGAGTTCCACGACTGGGACGACATCCGCGCCGAACTGCACGACGGCGACGGTGAGGCGCTAGCCGCTGAGCGGGC
GCGCACCGAGGCATGGGTCAGTGCATTCCATCTGGCTGAGGAGCGCAGGCGACTGGGGCTGACTCAGCGTCAGGTGGCCG
AGCTGATGGGTGTCTCCCCGGGCCGGGTGAGCCAGATCGAGAACGGTGATCTCGACGCCAACGAGGTCGCGACGCTGAGC
CGGTACGCCCGGGCGCTGGGTGCCCGCATGCGGATCATCTTCGACTATGGCAACGACCTCCGCCAGATCGTCTGA

Structures

Toxin

• AlphaFold DB: F8B1V8