Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3104831..3105558 Replicon chromosome
Accession NZ_CP102380
Organism Escherichia coli strain BM28

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NR349_RS15075 Protein ID WP_000550189.1
Coordinates 3105244..3105558 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NR349_RS15070 Protein ID WP_000560266.1
Coordinates 3104831..3105247 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR349_RS15060 (3099991) 3099991..3102342 + 2352 WP_000695487.1 alpha-glucosidase -
NR349_RS15065 (3102768) 3102768..3104786 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NR349_RS15070 (3104831) 3104831..3105247 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NR349_RS15075 (3105244) 3105244..3105558 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NR349_RS15080 (3105842) 3105842..3106978 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NR349_RS15085 (3107063) 3107063..3107566 + 504 WP_001333820.1 M48 family metallopeptidase -
NR349_RS15090 (3107643) 3107643..3108335 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
NR349_RS15095 (3108414) 3108414..3109400 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T253587 WP_000550189.1 NZ_CP102380:c3105558-3105244 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT253587 WP_000560266.1 NZ_CP102380:c3105247-3104831 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References