Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4228837..4229334 Replicon chromosome
Accession NZ_CP102379
Organism Escherichia coli strain BM28 lysU

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NR350_RS20450 Protein ID WP_001173343.1
Coordinates 4229161..4229334 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NR350_RS20445 Protein ID WP_000398619.1
Coordinates 4228837..4229133 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR350_RS20425 (4225568) 4225568..4226287 - 720 WP_000611288.1 two-component system response regulator DcuR -
NR350_RS20430 (4226284) 4226284..4227915 - 1632 WP_001216477.1 sensor histidine kinase -
NR350_RS20435 (4228096) 4228096..4228326 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NR350_RS20440 (4228338) 4228338..4228610 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
NR350_RS20445 (4228837) 4228837..4229133 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NR350_RS20450 (4229161) 4229161..4229334 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NR350_RS20455 (4229741) 4229741..4230400 + 660 WP_006286297.1 type A-1 chloramphenicol O-acetyltransferase -
NR350_RS20460 (4230739) 4230739..4232196 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island catA1 - 4228837..4237719 8882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T253555 WP_001173343.1 NZ_CP102379:4229161-4229334 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT253555 WP_000398619.1 NZ_CP102379:4228837-4229133 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References