Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3103495..3104222 Replicon chromosome
Accession NZ_CP102379
Organism Escherichia coli strain BM28 lysU

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NR350_RS15080 Protein ID WP_000550189.1
Coordinates 3103908..3104222 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NR350_RS15075 Protein ID WP_000560266.1
Coordinates 3103495..3103911 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR350_RS15065 (3098655) 3098655..3101006 + 2352 WP_000695487.1 alpha-glucosidase -
NR350_RS15070 (3101432) 3101432..3103450 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NR350_RS15075 (3103495) 3103495..3103911 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NR350_RS15080 (3103908) 3103908..3104222 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NR350_RS15085 (3104506) 3104506..3105642 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NR350_RS15090 (3105727) 3105727..3106230 + 504 WP_001333820.1 M48 family metallopeptidase -
NR350_RS15095 (3106307) 3106307..3106999 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
NR350_RS15100 (3107078) 3107078..3108064 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T253553 WP_000550189.1 NZ_CP102379:c3104222-3103908 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT253553 WP_000560266.1 NZ_CP102379:c3103911-3103495 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References